Research Articles
ReCiPe: The Essential Guide to Biosynthetic Route Assessment for Sustainable Pharma Development
This article provides a comprehensive guide to the ReCiPe (Resource Consumption and Environmental Impact) methodology, a leading life cycle assessment framework specifically tailored for evaluating the environmental and economic sustainability...
A Comprehensive Guide to RT-qPCR for Biosynthetic Gene Analysis: From Fundamentals to Advanced Validation in Drug Discovery
This article provides a complete methodological framework for utilizing Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) in the analysis of biosynthetic gene expression.
RODEO Algorithm: A Complete Guide for Accelerated RiPP Biosynthetic Gene Cluster Discovery
This comprehensive article explores RODEO (Rapid ORF Description and Evaluation Online), a pivotal computational tool for identifying Ribosomally synthesized and post-translationally modified peptide (RiPP) precursor peptides and their biosynthetic gene...
READRetro: The Ultimate Guide to AI-Powered Retrosynthesis for Drug Discovery Researchers
This comprehensive guide explores READRetro, a powerful retro web platform for computer-aided retrosynthesis planning.
PolE and PolF Genes: Key Enzymes in the Polyoxin Biosynthetic Pathway and Their Antifungal Potential
This article provides a comprehensive overview of the PolE and PolF genes, their crucial enzymatic roles in the biosynthesis of the nucleoside antibiotic polyoxin, and their implications for antifungal drug...
Screening Critical Factors: A Practical Guide to Plackett-Burman Design for Metabolic Pathway Optimization in Biomanufacturing
This comprehensive guide details the application of Plackett-Burman (PB) experimental design as a powerful, fractional-factorial screening tool for improving biogenesis and metabolic pathways in bioprocess development.
PACE Evolution: Revolutionizing Natural Product Drug Discovery with Phage-Assisted Continuous Evolution
This article provides a comprehensive guide for researchers on leveraging Phage-Assisted Continuous Evolution (PACE) to evolve and optimize enzymes for natural product biosynthesis.
Pep-PAT Assay: A Comprehensive Guide for Profiling Protein S-Acylation Substrates in Biomedical Research
This guide provides a detailed exploration of the Pep-PAT (Palmitoyl Acyltransferase-based Peptide Microarray) assay for identifying and characterizing protein S-acylation substrates.
Unlocking Nature's Medicine Chest: A Cutting-Edge Guide to PKS Gene Splitting for Enhanced Biosynthesis
This comprehensive guide provides researchers and drug development professionals with a strategic framework for leveraging polyketide synthase (PKS) gene splitting to overcome key bottlenecks in natural product biosynthesis.
Beyond Hydrolysis: Harnessing PBP-Type Thioesterases SurE and WolJ for Advanced Biocatalysis in Drug Discovery
This article provides a comprehensive overview of SurE and WolJ, two underutilized PBP-type thioesterases, exploring their unique structural and catalytic mechanisms beyond their traditional roles in cell wall recycling.