Research Articles
Beyond Hydrolysis: Harnessing PBP-Type Thioesterases SurE and WolJ for Advanced Biocatalysis in Drug Discovery
This article provides a comprehensive overview of SurE and WolJ, two underutilized PBP-type thioesterases, exploring their unique structural and catalytic mechanisms beyond their traditional roles in cell wall recycling.
Engineering PBP-Type Teichoic Acid Substrates: A Strategic Guide for Overcoming Beta-Lactam Resistance
This comprehensive review addresses the critical challenge of beta-lactam antibiotic resistance by exploring the engineering of PBP-type teichoic acid (TE) substrates.
OrthoRep: A Complete Guide to In Vivo Continuous Evolution for Protein Engineering and Drug Discovery
This comprehensive guide explores the OrthoRep in vivo evolution platform, a revolutionary system for continuous, error-prone replication of orthogonal DNA plasmids in yeast.
NRPS vs RiPP Pathways: A Comparative Guide for Drug Discovery Scientists
This article provides a comprehensive, comparative analysis of Nonribosomal Peptide Synthetase (NRPS) and Ribosomally synthesized and Post-translationally modified Peptide (RiPP) biosynthetic pathways.
NRPS Thioesterase Domains: Unveiling the Catalytic Machinery of Product Release in Natural Product Biosynthesis
This article provides a comprehensive analysis of the Nonribosomal Peptide Synthetase (NRPS) Thioesterase (TE) domain, the critical gatekeeper responsible for product release, cyclization, and macrocyclization.
Reprogramming NRPS Assembly Lines: Engineering Nonribosomal Peptide Synthetases for Novel Therapeutics
This comprehensive article explores the cutting-edge field of nonribosomal peptide synthetase (NRPS) repurposing for novel chemical production.
Decoding Nature's Assembly Line: A Comprehensive Guide to NMR-Based Structure Elucidation of Nonribosomal Peptides (NRPs)
This article provides a comprehensive framework for the nuclear magnetic resonance (NMR) spectroscopy-driven characterization and structure elucidation of nonribosomal peptides (NRPs), a critical class of bioactive natural products.
Unlocking Natural Product Discovery: A Comprehensive Guide to NRPS Phylogenetic Analysis and Conserved Gene Cluster Prediction
This article provides a comprehensive guide for researchers and industry professionals on the phylogenetic analysis of Non-Ribosomal Peptide Synthetase (NRPS) gene clusters.
Silent No More: Unlocking Novel Natural Products by Reactivating Dormant NRPS Gene Clusters
This article provides a comprehensive guide for researchers and drug discovery professionals on the strategies and methodologies for reactivating cryptic Nonribosomal Peptide Synthetase (NRPS) gene clusters.
From Molecule to Machine: Predicting NRPS Biosynthetic Modules from Chemical Structure Using AI and Bioinformatics
This comprehensive guide for researchers and drug discovery professionals explores the frontier of predicting Nonribosomal Peptide Synthetase (NRPS) modules directly from chemical structures.